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Comparing the efficiency of six clearing methods in developing seeds of Arabidopsis thaliana
KEY MESSAGE: ClearSee alpha and FAST9 were optimized for imaging Arabidopsis seeds up to the torpedo stages. The methods preserve the fluorescence of reporter proteins and seed shape, allowing phenotyping embryos in intact seeds. ABSTRACT: Tissue clearing methods eliminate the need for sectioning, t...
Autores principales: | Attuluri, Venkata Pardha Saradhi, Sánchez López, Juan Francisco, Maier, Lukáš, Paruch, Kamil, Robert, Hélène S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705463/ https://www.ncbi.nlm.nih.gov/pubmed/36378346 http://dx.doi.org/10.1007/s00497-022-00453-4 |
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