Construction, Investigation and Application of TEV Protease Variants with Improved Oxidative Stability

Tobacco etch virus protease (TEVp) is a useful tool for removing fusion tags, but wild-type TEVp is less stable under oxidized redox state. In this work, we introduced and combined C19S, C110S and C130S into TEVp variants containing T17S, L56V, N68D, I77V and S135G to improve protein solubility, and...

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Detalles Bibliográficos
Autores principales: Bayar, Enkhtuya, Ren, Yuanyuan, Chen, Yinghua, Hu, Yafang, Zhang, Shuncheng, Yu, Xuelian, Fan, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2021
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705859/
https://www.ncbi.nlm.nih.gov/pubmed/34528919
http://dx.doi.org/10.4014/jmb.2106.06075

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705859/
https://www.ncbi.nlm.nih.gov/pubmed/34528919
http://dx.doi.org/10.4014/jmb.2106.06075

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