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SARS-CoV-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual

The milder clinical manifestations of Omicron infection relative to pre-Omicron SARS CoV-2 raises the possibility that extensive evolution results in reduced pathogenicity. To test this hypothesis, we quantified induction of cell fusion and cell death in SARS CoV-2 evolved from ancestral virus durin...

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Autores principales: Lustig, Gila, Ganga, Yashica, Rodel, Hylton, Tegally, Houriiyah, Jackson, Laurelle, Cele, Sandile, Khan, Khadija, Jule, Zesuliwe, Reedoy, Kajal, Karim, Farina, Bernstein, Mallory, Moosa, Mahomed-Yunus S., Archary, Derseree, de Oliveira, Tulio, Lessells, Richard, Abdool Karim, Salim S., Sigal, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709797/
https://www.ncbi.nlm.nih.gov/pubmed/36451879
http://dx.doi.org/10.1101/2022.11.23.22282673
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author Lustig, Gila
Ganga, Yashica
Rodel, Hylton
Tegally, Houriiyah
Jackson, Laurelle
Cele, Sandile
Khan, Khadija
Jule, Zesuliwe
Reedoy, Kajal
Karim, Farina
Bernstein, Mallory
Moosa, Mahomed-Yunus S.
Archary, Derseree
de Oliveira, Tulio
Lessells, Richard
Abdool Karim, Salim S.
Sigal, Alex
author_facet Lustig, Gila
Ganga, Yashica
Rodel, Hylton
Tegally, Houriiyah
Jackson, Laurelle
Cele, Sandile
Khan, Khadija
Jule, Zesuliwe
Reedoy, Kajal
Karim, Farina
Bernstein, Mallory
Moosa, Mahomed-Yunus S.
Archary, Derseree
de Oliveira, Tulio
Lessells, Richard
Abdool Karim, Salim S.
Sigal, Alex
author_sort Lustig, Gila
collection PubMed
description The milder clinical manifestations of Omicron infection relative to pre-Omicron SARS CoV-2 raises the possibility that extensive evolution results in reduced pathogenicity. To test this hypothesis, we quantified induction of cell fusion and cell death in SARS CoV-2 evolved from ancestral virus during long-term infection. Both cell fusion and death were reduced in Omicron BA.1 infection relative to ancestral virus. Evolved virus was isolated at different times during a 6-month infection in an immunosuppressed individual with advanced HIV disease. The virus isolated 16 days post-reported symptom onset induced fusogenicity and cell death at levels similar to BA.1. However, fusogenicity was increased in virus isolated at 6 months post-symptoms to levels intermediate between BA.1 and ancestral SARS-CoV-2. Similarly, infected cell death showed a graded increase from earlier to later isolates. These results may indicate that, at least by the cellular measures used here, evolution in long-term infection does not necessarily attenuate the virus.
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spelling pubmed-97097972022-12-15 SARS-CoV-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual Lustig, Gila Ganga, Yashica Rodel, Hylton Tegally, Houriiyah Jackson, Laurelle Cele, Sandile Khan, Khadija Jule, Zesuliwe Reedoy, Kajal Karim, Farina Bernstein, Mallory Moosa, Mahomed-Yunus S. Archary, Derseree de Oliveira, Tulio Lessells, Richard Abdool Karim, Salim S. Sigal, Alex medRxiv Article The milder clinical manifestations of Omicron infection relative to pre-Omicron SARS CoV-2 raises the possibility that extensive evolution results in reduced pathogenicity. To test this hypothesis, we quantified induction of cell fusion and cell death in SARS CoV-2 evolved from ancestral virus during long-term infection. Both cell fusion and death were reduced in Omicron BA.1 infection relative to ancestral virus. Evolved virus was isolated at different times during a 6-month infection in an immunosuppressed individual with advanced HIV disease. The virus isolated 16 days post-reported symptom onset induced fusogenicity and cell death at levels similar to BA.1. However, fusogenicity was increased in virus isolated at 6 months post-symptoms to levels intermediate between BA.1 and ancestral SARS-CoV-2. Similarly, infected cell death showed a graded increase from earlier to later isolates. These results may indicate that, at least by the cellular measures used here, evolution in long-term infection does not necessarily attenuate the virus. Cold Spring Harbor Laboratory 2022-11-30 /pmc/articles/PMC9709797/ /pubmed/36451879 http://dx.doi.org/10.1101/2022.11.23.22282673 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Lustig, Gila
Ganga, Yashica
Rodel, Hylton
Tegally, Houriiyah
Jackson, Laurelle
Cele, Sandile
Khan, Khadija
Jule, Zesuliwe
Reedoy, Kajal
Karim, Farina
Bernstein, Mallory
Moosa, Mahomed-Yunus S.
Archary, Derseree
de Oliveira, Tulio
Lessells, Richard
Abdool Karim, Salim S.
Sigal, Alex
SARS-CoV-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual
title SARS-CoV-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual
title_full SARS-CoV-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual
title_fullStr SARS-CoV-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual
title_full_unstemmed SARS-CoV-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual
title_short SARS-CoV-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual
title_sort sars-cov-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709797/
https://www.ncbi.nlm.nih.gov/pubmed/36451879
http://dx.doi.org/10.1101/2022.11.23.22282673
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