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Pure shift amide detection in conventional and TROSY-type experiments of (13)C,(15)N-labeled proteins
Large coupling networks in uniformly (13)C,(15)N-labeled biomolecules induce broad multiplets that even in flexible proteins are frequently not recognized as such. The reason is that given multiplets typically consist of a large number of individual resonances that result in a single broad line, in...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712348/ https://www.ncbi.nlm.nih.gov/pubmed/36399207 http://dx.doi.org/10.1007/s10858-022-00406-z |