Self-cleaving guide RNAs enable pharmacological selection of precise gene editing events in vivo

Expression of guide RNAs in the CRISPR/Cas9 system typically requires the use of RNA polymerase III promoters, which are not cell-type specific. Flanking the gRNA with self-cleaving ribozyme motifs to create a self-cleaving gRNA overcomes this limitation. Here, we use self-cleaving gRNAs to create d...

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Detalles Bibliográficos
Autores principales: Tiyaboonchai, Amita, Vonada, Anne, Posey, Jeffrey, Pelz, Carl, Wakefield, Leslie, Grompe, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712609/
https://www.ncbi.nlm.nih.gov/pubmed/36450762
http://dx.doi.org/10.1038/s41467-022-35097-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712609/
https://www.ncbi.nlm.nih.gov/pubmed/36450762
http://dx.doi.org/10.1038/s41467-022-35097-5

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