Target-enriched nanopore sequencing and de novo assembly reveals co-occurrences of complex on-target genomic rearrangements induced by CRISPR-Cas9 in human cells

The CRISPR-Cas9 system is widely used to permanently delete genomic regions via dual guide RNAs. Genomic rearrangements induced by CRISPR-Cas9 can occur, but continuous technical developments make it possible to characterize complex on-target effects. We combined an innovative droplet-based target e...

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Detalles Bibliográficos
Autores principales: Geng, Keyi, Merino, Lara G., Wedemann, Linda, Martens, Aniek, Sobota, Małgorzata, Sanchez, Yerma P., Søndergaard, Jonas Nørskov, White, Robert J., Kutter, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712622/
https://www.ncbi.nlm.nih.gov/pubmed/36180232
http://dx.doi.org/10.1101/gr.276901.122

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712622/
https://www.ncbi.nlm.nih.gov/pubmed/36180232
http://dx.doi.org/10.1101/gr.276901.122

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