A cryogenic, coincident fluorescence, electron, and ion beam microscope

Cryogenic electron tomography (cryo-ET) combined with subtomogram averaging, allows in situ visualization and structure determination of macromolecular complexes at subnanometre resolution. Cryogenic focused ion beam (cryo-FIB) micromachining is used to prepare a thin lamella-shaped sample out of a...

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Detalles Bibliográficos
Autores principales: Boltje, Daan B, Hoogenboom, Jacob P, Jakobi, Arjen J, Jensen, Grant J, Jonker, Caspar TH, Kaag, Max J, Koster, Abraham J, Last, Mart GF, de Agrela Pinto, Cecilia, Plitzko, Jürgen M, Raunser, Stefan, Tacke, Sebastian, Wang, Zhexin, van der Wee, Ernest B, Wepf, Roger, den Hoedt, Sander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Structural Biology and Molecular Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714966/
https://www.ncbi.nlm.nih.gov/pubmed/36305590
http://dx.doi.org/10.7554/eLife.82891

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714966/
https://www.ncbi.nlm.nih.gov/pubmed/36305590
http://dx.doi.org/10.7554/eLife.82891

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