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The Combination of Electrochemistry and Microfluidic Technology in Drug Metabolism Studies

Drugs are metabolized within the liver (pH 7.4) by phase I and phase II metabolism. During the process, reactive metabolites can be formed that react covalently with biomolecules and induce toxicity. Identifying and detecting reactive metabolites is an important part of drug development. Preclinical...

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Autores principales: Grint, Isobel, Crea, Francesco, Vasiliadou, Rafaela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716038/
https://www.ncbi.nlm.nih.gov/pubmed/36166688
http://dx.doi.org/10.1002/open.202200100
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author Grint, Isobel
Crea, Francesco
Vasiliadou, Rafaela
author_facet Grint, Isobel
Crea, Francesco
Vasiliadou, Rafaela
author_sort Grint, Isobel
collection PubMed
description Drugs are metabolized within the liver (pH 7.4) by phase I and phase II metabolism. During the process, reactive metabolites can be formed that react covalently with biomolecules and induce toxicity. Identifying and detecting reactive metabolites is an important part of drug development. Preclinical and clinical investigations are conducted to assess the toxicity and safety of a new drug candidate. Electrochemistry coupled to mass spectrometry is an ideal complementary technique to the current preclinical studies, a pure instrumental approach without any purification steps and tedious protocols. The combination of microfluidics with electrochemistry towards the mimicry of drug metabolism offers portability, low volume of reagents and faster reaction times. This review explores the development of microfluidic electrochemical cells for mimicking drug metabolism.
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spelling pubmed-97160382022-12-05 The Combination of Electrochemistry and Microfluidic Technology in Drug Metabolism Studies Grint, Isobel Crea, Francesco Vasiliadou, Rafaela ChemistryOpen Reviews Drugs are metabolized within the liver (pH 7.4) by phase I and phase II metabolism. During the process, reactive metabolites can be formed that react covalently with biomolecules and induce toxicity. Identifying and detecting reactive metabolites is an important part of drug development. Preclinical and clinical investigations are conducted to assess the toxicity and safety of a new drug candidate. Electrochemistry coupled to mass spectrometry is an ideal complementary technique to the current preclinical studies, a pure instrumental approach without any purification steps and tedious protocols. The combination of microfluidics with electrochemistry towards the mimicry of drug metabolism offers portability, low volume of reagents and faster reaction times. This review explores the development of microfluidic electrochemical cells for mimicking drug metabolism. John Wiley and Sons Inc. 2022-09-27 /pmc/articles/PMC9716038/ /pubmed/36166688 http://dx.doi.org/10.1002/open.202200100 Text en © 2022 The Authors. Published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Reviews
Grint, Isobel
Crea, Francesco
Vasiliadou, Rafaela
The Combination of Electrochemistry and Microfluidic Technology in Drug Metabolism Studies
title The Combination of Electrochemistry and Microfluidic Technology in Drug Metabolism Studies
title_full The Combination of Electrochemistry and Microfluidic Technology in Drug Metabolism Studies
title_fullStr The Combination of Electrochemistry and Microfluidic Technology in Drug Metabolism Studies
title_full_unstemmed The Combination of Electrochemistry and Microfluidic Technology in Drug Metabolism Studies
title_short The Combination of Electrochemistry and Microfluidic Technology in Drug Metabolism Studies
title_sort combination of electrochemistry and microfluidic technology in drug metabolism studies
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716038/
https://www.ncbi.nlm.nih.gov/pubmed/36166688
http://dx.doi.org/10.1002/open.202200100
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