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A two-stage digestion of whole murine knee joints for single-cell RNA sequencing

OBJECTIVE: Single-cell RNA sequencing (scRNA-seq) is a powerful technology that can be applied to the cells populating the whole knee in the study of joint pathology. The knee contains cells embedded in hard structural tissues, cells in softer tissues and membranes, and immune cells. This creates a...

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Detalles Bibliográficos
Autores principales: Leale, Dustin M., Li, Linan, Settles, Matthew L., Mitchell, Keith, Froenicke, Lutz, Yik, Jasper H.N., Haudenschild, Dominik R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718180/
https://www.ncbi.nlm.nih.gov/pubmed/36474787
http://dx.doi.org/10.1016/j.ocarto.2022.100321