Chemically stable fluorescent proteins for advanced microscopy

We report the rational engineering of a remarkably stable yellow fluorescent protein (YFP), ‘hyperfolder YFP’ (hfYFP), that withstands chaotropic conditions that denature most biological structures within seconds, including superfolder green fluorescent protein (GFP). hfYFP contains no cysteines, is...

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Detalles Bibliográficos
Autores principales: Campbell, Benjamin C., Paez-Segala, Maria G., Looger, Loren L., Petsko, Gregory A., Liu, Ce Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718679/
https://www.ncbi.nlm.nih.gov/pubmed/36344833
http://dx.doi.org/10.1038/s41592-022-01660-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718679/
https://www.ncbi.nlm.nih.gov/pubmed/36344833
http://dx.doi.org/10.1038/s41592-022-01660-7

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