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A growth selection system for the directed evolution of amine-forming or converting enzymes

Fast screening of enzyme variants is crucial for tailoring biocatalysts for the asymmetric synthesis of non-natural chiral chemicals, such as amines. However, most existing screening methods either are limited by the throughput or require specialized equipment. Herein, we report a simple, high-throu...

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Detalles Bibliográficos
Autores principales: Wu, Shuke, Xiang, Chao, Zhou, Yi, Khan, Mohammad Saiful Hasan, Liu, Weidong, Feiler, Christian G., Wei, Ren, Weber, Gert, Höhne, Matthias, Bornscheuer, Uwe T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718777/
https://www.ncbi.nlm.nih.gov/pubmed/36460668
http://dx.doi.org/10.1038/s41467-022-35228-y