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Effect of kokumi taste-active γ-glutamyl peptides on amiloride-sensitive epithelial Na(+) channels in rat fungiform taste cells
Kokumi taste-active compounds enhance salty taste perception. In animal models, sodium (salt) detection is mediated by the amiloride-sensitive epithelial sodium channel, ENaC. This ion channel works as a sodium receptor in the so-called sodium-taste cells. It is not known whether kokumi taste substa...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719077/ https://www.ncbi.nlm.nih.gov/pubmed/36471792 http://dx.doi.org/10.1016/j.bbrep.2022.101400 |
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author | Bigiani, Albertino Rhyu, MeeRa |
author_facet | Bigiani, Albertino Rhyu, MeeRa |
author_sort | Bigiani, Albertino |
collection | PubMed |
description | Kokumi taste-active compounds enhance salty taste perception. In animal models, sodium (salt) detection is mediated by the amiloride-sensitive epithelial sodium channel, ENaC. This ion channel works as a sodium receptor in the so-called sodium-taste cells. It is not known whether kokumi taste substances are able to affect the activity of functional ENaCs in these cells. Here, we use the patch-clamp technique to study the effect of kokumi-active tripeptides, glutathione (GSH) and γ-glutamyl-valyl-glycine (EVG), on the ENaC-mediated membrane current in rat fungiform sodium-taste cells. GSH and EVG reduced slightly this current and the effect disappeared in the presence of amiloride, a specific ENaC blocker. No effect on membrane current was detected in other taste cells (Type II and Type III cells) that do not express functional ENaC. Our findings suggest that the enhancing effect of kokumi taste-active γ-glutamyl peptides on salt reception is not explained by an increase in the activity of ENaC. |
format | Online Article Text |
id | pubmed-9719077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-97190772022-12-04 Effect of kokumi taste-active γ-glutamyl peptides on amiloride-sensitive epithelial Na(+) channels in rat fungiform taste cells Bigiani, Albertino Rhyu, MeeRa Biochem Biophys Rep Research Article Kokumi taste-active compounds enhance salty taste perception. In animal models, sodium (salt) detection is mediated by the amiloride-sensitive epithelial sodium channel, ENaC. This ion channel works as a sodium receptor in the so-called sodium-taste cells. It is not known whether kokumi taste substances are able to affect the activity of functional ENaCs in these cells. Here, we use the patch-clamp technique to study the effect of kokumi-active tripeptides, glutathione (GSH) and γ-glutamyl-valyl-glycine (EVG), on the ENaC-mediated membrane current in rat fungiform sodium-taste cells. GSH and EVG reduced slightly this current and the effect disappeared in the presence of amiloride, a specific ENaC blocker. No effect on membrane current was detected in other taste cells (Type II and Type III cells) that do not express functional ENaC. Our findings suggest that the enhancing effect of kokumi taste-active γ-glutamyl peptides on salt reception is not explained by an increase in the activity of ENaC. Elsevier 2022-12-01 /pmc/articles/PMC9719077/ /pubmed/36471792 http://dx.doi.org/10.1016/j.bbrep.2022.101400 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Bigiani, Albertino Rhyu, MeeRa Effect of kokumi taste-active γ-glutamyl peptides on amiloride-sensitive epithelial Na(+) channels in rat fungiform taste cells |
title | Effect of kokumi taste-active γ-glutamyl peptides on amiloride-sensitive epithelial Na(+) channels in rat fungiform taste cells |
title_full | Effect of kokumi taste-active γ-glutamyl peptides on amiloride-sensitive epithelial Na(+) channels in rat fungiform taste cells |
title_fullStr | Effect of kokumi taste-active γ-glutamyl peptides on amiloride-sensitive epithelial Na(+) channels in rat fungiform taste cells |
title_full_unstemmed | Effect of kokumi taste-active γ-glutamyl peptides on amiloride-sensitive epithelial Na(+) channels in rat fungiform taste cells |
title_short | Effect of kokumi taste-active γ-glutamyl peptides on amiloride-sensitive epithelial Na(+) channels in rat fungiform taste cells |
title_sort | effect of kokumi taste-active γ-glutamyl peptides on amiloride-sensitive epithelial na(+) channels in rat fungiform taste cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719077/ https://www.ncbi.nlm.nih.gov/pubmed/36471792 http://dx.doi.org/10.1016/j.bbrep.2022.101400 |
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