Directed Evolution of Soluble α-1,2-Fucosyltransferase Using Kanamycin Resistance Protein as a Phenotypic Reporter for Efficient Production of 2'-Fucosyllactose

2'-Fucosyllactose (2'-FL), the most abundant fucosylated oligosaccharide in human milk, has multiple beneficial effects on human health. However, its biosynthesis by metabolically engineered Escherichia coli is often hampered owing to the insolubility and instability of α-1,2-fucosyltransf...

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Detalles Bibliográficos
Autores principales: Shin, Jonghyeok, Kim, Seungjoo, Park, Wonbeom, Jin, Kyoung Chan, Kim, Sun-Ki, Kweon, Dae-Hyuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2022
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9720067/
https://www.ncbi.nlm.nih.gov/pubmed/36437520
http://dx.doi.org/10.4014/jmb.2209.09018

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9720067/
https://www.ncbi.nlm.nih.gov/pubmed/36437520
http://dx.doi.org/10.4014/jmb.2209.09018

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