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Enhanced protein–protein interaction network construction promoted by in vivo cross-linking with acid-cleavable click-chemistry enrichment

Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enables global profiling of protein interactome with direct interaction interfaces in complex biological systems. The alkyne-tagged enrichable cross-linkers are preferred to improve the coverage of low-abu...

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Detalles Bibliográficos
Autores principales: Zhao, Lili, Zhong, Bowen, An, Yuxin, Zhang, Weijie, Gao, Hang, Zhang, Xiaodan, Liang, Zhen, Zhang, Yukui, Zhao, Qun, Zhang, Lihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9720147/
https://www.ncbi.nlm.nih.gov/pubmed/36479438
http://dx.doi.org/10.3389/fchem.2022.994572