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Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human

Here we describe a multiplex chromogenic immunohistochemistry platform to stain and analyze two markers in paraffin tissue sections from mouse or human. The basis of the protocol is a series of stripping and re-probing steps with subsequent image analysis, which allows the user to perform multiplex...

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Detalles Bibliográficos
Autores principales: Maiques, Oscar, Sanz-Moreno, Victoria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9720345/
https://www.ncbi.nlm.nih.gov/pubmed/36595909
http://dx.doi.org/10.1016/j.xpro.2022.101879