Specific and efficient gene knockout and overexpression in mouse interscapular brown adipocytes in vivo

The classical Cre-LoxP system is time consuming. Here we detail a protocol that leverages Rosa26-LSL-Cas9;Adiponectin-Cre mice to restrict Cas9 expression in adipocytes. This enables specific deletion of target genes in brown adipocytes within 6 weeks by local injection of AAV-sgRNA into interscapul...

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Detalles Bibliográficos
Autores principales: Xue, Kaili, Wu, Dongmei, Qiu, Yifu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722717/
https://www.ncbi.nlm.nih.gov/pubmed/36595932
http://dx.doi.org/10.1016/j.xpro.2022.101895

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722717/
https://www.ncbi.nlm.nih.gov/pubmed/36595932
http://dx.doi.org/10.1016/j.xpro.2022.101895

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