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Transcript variants of long-chain acyl-CoA synthase 1 have distinct roles in sheep lipid metabolism

Mutton has recently been identified to be a consumer favorite, and intermuscular fat is the key factor in determining meat tenderness. Long-chain acyl-CoA synthetase 1 (ACSL1) is a vital subtype of the ACSL family that is involved in the synthesis of lipids from acyl-CoA and the oxidation of fatty a...

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Autores principales: Cao, Yang, Yu, Yongsheng, Zhang, Lichun, Liu, Yu, Zheng, Kaizhi, Wang, Sutian, Jin, Haiguo, Liu, Lixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723241/
https://www.ncbi.nlm.nih.gov/pubmed/36482895
http://dx.doi.org/10.3389/fgene.2022.1021103
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author Cao, Yang
Yu, Yongsheng
Zhang, Lichun
Liu, Yu
Zheng, Kaizhi
Wang, Sutian
Jin, Haiguo
Liu, Lixiang
Cao, Yang
author_facet Cao, Yang
Yu, Yongsheng
Zhang, Lichun
Liu, Yu
Zheng, Kaizhi
Wang, Sutian
Jin, Haiguo
Liu, Lixiang
Cao, Yang
author_sort Cao, Yang
collection PubMed
description Mutton has recently been identified to be a consumer favorite, and intermuscular fat is the key factor in determining meat tenderness. Long-chain acyl-CoA synthetase 1 (ACSL1) is a vital subtype of the ACSL family that is involved in the synthesis of lipids from acyl-CoA and the oxidation of fatty acids. The amplification of the ACSL1 gene using rapid amplification of cDNA ends revealed that the alternative polyadenylation (APA) results in two transcripts of the ACSL1 gene. Exon 18 had premature termination, resulting in a shorter CDS region. In this study, the existence of two transcripts of varying lengths translated normally and designated ACSL1-a and ACSL1-b was confirmed. Overexpression of ACSL1-a can promote the synthesis of an intracellular diglyceride, while ACSL1-b can promote triglyceride synthesis. The transfection of ACSL1 shRNA knocks down both the transcripts, the triglyceride content was significantly reduced after differentiation and induction; and lipidome sequencing results exhibited a significant decrease in 14–22 carbon triglyceride metabolites. The results of the present study indicated that the ACSL1 gene played a crucial role in the synthesis of triglycerides. Furthermore, the two transcripts involved in various interactions in the triglyceride synthesis process may be the topic of interest for future research and provide a more theoretical basis for sheep breeding.
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spelling pubmed-97232412022-12-07 Transcript variants of long-chain acyl-CoA synthase 1 have distinct roles in sheep lipid metabolism Cao, Yang Yu, Yongsheng Zhang, Lichun Liu, Yu Zheng, Kaizhi Wang, Sutian Jin, Haiguo Liu, Lixiang Cao, Yang Front Genet Genetics Mutton has recently been identified to be a consumer favorite, and intermuscular fat is the key factor in determining meat tenderness. Long-chain acyl-CoA synthetase 1 (ACSL1) is a vital subtype of the ACSL family that is involved in the synthesis of lipids from acyl-CoA and the oxidation of fatty acids. The amplification of the ACSL1 gene using rapid amplification of cDNA ends revealed that the alternative polyadenylation (APA) results in two transcripts of the ACSL1 gene. Exon 18 had premature termination, resulting in a shorter CDS region. In this study, the existence of two transcripts of varying lengths translated normally and designated ACSL1-a and ACSL1-b was confirmed. Overexpression of ACSL1-a can promote the synthesis of an intracellular diglyceride, while ACSL1-b can promote triglyceride synthesis. The transfection of ACSL1 shRNA knocks down both the transcripts, the triglyceride content was significantly reduced after differentiation and induction; and lipidome sequencing results exhibited a significant decrease in 14–22 carbon triglyceride metabolites. The results of the present study indicated that the ACSL1 gene played a crucial role in the synthesis of triglycerides. Furthermore, the two transcripts involved in various interactions in the triglyceride synthesis process may be the topic of interest for future research and provide a more theoretical basis for sheep breeding. Frontiers Media S.A. 2022-11-22 /pmc/articles/PMC9723241/ /pubmed/36482895 http://dx.doi.org/10.3389/fgene.2022.1021103 Text en Copyright © 2022 Cao, Yu, Zhang, Liu, Zheng, Wang, Jin, Liu and Cao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Cao, Yang
Yu, Yongsheng
Zhang, Lichun
Liu, Yu
Zheng, Kaizhi
Wang, Sutian
Jin, Haiguo
Liu, Lixiang
Cao, Yang
Transcript variants of long-chain acyl-CoA synthase 1 have distinct roles in sheep lipid metabolism
title Transcript variants of long-chain acyl-CoA synthase 1 have distinct roles in sheep lipid metabolism
title_full Transcript variants of long-chain acyl-CoA synthase 1 have distinct roles in sheep lipid metabolism
title_fullStr Transcript variants of long-chain acyl-CoA synthase 1 have distinct roles in sheep lipid metabolism
title_full_unstemmed Transcript variants of long-chain acyl-CoA synthase 1 have distinct roles in sheep lipid metabolism
title_short Transcript variants of long-chain acyl-CoA synthase 1 have distinct roles in sheep lipid metabolism
title_sort transcript variants of long-chain acyl-coa synthase 1 have distinct roles in sheep lipid metabolism
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723241/
https://www.ncbi.nlm.nih.gov/pubmed/36482895
http://dx.doi.org/10.3389/fgene.2022.1021103
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