A new family of CRISPR‐type V nucleases with C‐rich PAM recognition

Most CRISPR‐type V nucleases are stimulated to cleave double‐stranded (ds) DNA targets by a T‐rich PAM, which restricts their targeting range. Here, we identify and characterize a new family of type V RNA‐guided nuclease, Cas12l, that exclusively recognizes a C‐rich (5'‐CCY‐3′) PAM. The organiz...

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Detalles Bibliográficos
Autores principales: Urbaitis, Tomas, Gasiunas, Giedrius, Young, Joshua K, Hou, Zhenglin, Paulraj, Sushmitha, Godliauskaite, Egle, Juskeviciene, Mantvyda M, Stitilyte, Migle, Jasnauskaite, Monika, Mabuchi, Megumu, Robb, G Brett, Siksnys, Virginijus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724661/
https://www.ncbi.nlm.nih.gov/pubmed/36268581
http://dx.doi.org/10.15252/embr.202255481

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724661/
https://www.ncbi.nlm.nih.gov/pubmed/36268581
http://dx.doi.org/10.15252/embr.202255481

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