Design of novel cyanovirin-N variants by modulation of binding dynamics through distal mutations

We develop integrated co-evolution and dynamic coupling (ICDC) approach to identify, mutate, and assess distal sites to modulate function. We validate the approach first by analyzing the existing mutational fitness data of TEM-1 β-lactamase and show that allosteric positions co-evolved and dynamical...

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Detalles Bibliográficos
Autores principales: Kazan, I Can, Sharma, Prerna, Rahman, Mohammad Imtiazur, Bobkov, Andrey, Fromme, Raimund, Ghirlanda, Giovanna, Ozkan, S Banu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Structural Biology and Molecular Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9725752/
https://www.ncbi.nlm.nih.gov/pubmed/36472898
http://dx.doi.org/10.7554/eLife.67474

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9725752/
https://www.ncbi.nlm.nih.gov/pubmed/36472898
http://dx.doi.org/10.7554/eLife.67474

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