Fluorescently tagged nanobodies and NanoBRET to study ligand-binding and agonist-induced conformational changes of full-length EGFR expressed in living cells

INTRODUCTION: The Epidermal Growth Factor Receptor is a member of the Erb receptor tyrosine kinase family. It binds several ligands including EGF, betacellulin (BTC) and TGF-α, controls cellular proliferation and invasion and is overexpressed in various cancer types. Nanobodies (VHHs) are the antige...

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Detalles Bibliográficos
Autores principales: Comez, Dehan, Glenn, Jacqueline, Anbuhl, Stephanie M., Heukers, Raimond, Smit, Martine J., Hill, Stephen J., Kilpatrick, Laura E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726709/
https://www.ncbi.nlm.nih.gov/pubmed/36505413
http://dx.doi.org/10.3389/fimmu.2022.1006718

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726709/
https://www.ncbi.nlm.nih.gov/pubmed/36505413
http://dx.doi.org/10.3389/fimmu.2022.1006718

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