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An Optimized Circular Polymerase Extension Reaction-based Method for Functional Analysis of SARS-CoV-2
Reverse genetics systems have been crucial for studying specific viral genes and their relevance in the virus lifecycle, and become important tools for the rational attenuation of viruses and thereby for vaccine design. Recent rapid progress has been made in the establishment of reverse genetics sys...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727755/ https://www.ncbi.nlm.nih.gov/pubmed/36482966 http://dx.doi.org/10.1101/2022.11.26.518005 |