Complete Combinatorial Mutational Enumeration of a protein functional site enables sequence-landscape mapping and identifies highly-mutated variants that retain activity

Understanding how proteins evolve under selective pressure is a longstanding challenge. The immensity of the search space has limited efforts to systematically evaluate the impact of multiple simultaneous mutations, so mutations have typically been assessed individually. However, epistasis, or the w...

Descripción completa

Detalles Bibliográficos
Autores principales: Colom, Mireia Solà, Vucinic, Jelena, Adolf-Bryfogle, Jared, Bowman, James W., Verel, Sébastien, Moczygemba, Isabelle, Schiex, Thomas, Simoncini, David, Bahl, Christopher D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727770/
https://www.ncbi.nlm.nih.gov/pubmed/36482980
http://dx.doi.org/10.21203/rs.3.rs-2248327/v2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727770/
https://www.ncbi.nlm.nih.gov/pubmed/36482980
http://dx.doi.org/10.21203/rs.3.rs-2248327/v2

Ejemplares similares