Single-Base Genome Editing in Corynebacterium glutamicum with the Help of Negative Selection by Target-Mismatched CRISPR/Cpf1

CRISPR/Cpf1 has emerged as a new CRISPR-based genome editing tool because, in comparison with CRIPSR/Cas9, it has a different T-rich PAM sequence to expand the target DNA sequence. Single-base editing in the microbial genome can be facilitated by oligonucleotide-directed mutagenesis (ODM) followed b...

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Detalles Bibliográficos
Autores principales: Kim, Hyun Ju, Oh, Se Young, Lee, Sang Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Microbiology and Biotechnology 2020
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728170/
https://www.ncbi.nlm.nih.gov/pubmed/32807756
http://dx.doi.org/10.4014/jmb.2006.06036

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728170/
https://www.ncbi.nlm.nih.gov/pubmed/32807756
http://dx.doi.org/10.4014/jmb.2006.06036

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