Addition of an N-Terminal Poly-Glutamate Fusion Tag Improves Solubility and Production of Recombinant TAT-Cre Recombinase in Escherichia coli

Cre recombinase is widely used to manipulate DNA sequences for both in vitro and in vivo research. Attachment of a trans-activator of transcription (TAT) sequence to Cre allows TATCre to penetrate the cell membrane, and the addition of a nuclear localization signal (NLS) helps the enzyme to transloc...

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Detalles Bibliográficos
Autores principales: Kim, A-Hyeon, Lee, Soohyun, Jeon, Suwon, Kim, Goon-Tae, Lee, Eun Jig, Kim, Daham, Kim, Younggyu, Park, Tae-Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Microbiology and Biotechnology 2020
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728232/
https://www.ncbi.nlm.nih.gov/pubmed/31693834
http://dx.doi.org/10.4014/jmb.1909.09028

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728232/
https://www.ncbi.nlm.nih.gov/pubmed/31693834
http://dx.doi.org/10.4014/jmb.1909.09028

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