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Cell Surface Display of Poly(3-hydroxybutyrate) Depolymerase and its Application

We have expressed extracellular poly(3-hydroxybutyrate) (PHB) depolymerase of Ralstonia pickettii T1 on the Escherichia coli surface using Pseudomonas OprF protein as a fusion partner by C-terminal deletion-fusion strategy. Surface display of depolymerase was confirmed by flow cytometry, immunofluor...

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Detalles Bibliográficos
Autores principales: Lee, Seung Hwan, Lee, Sang Yup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Microbiology and Biotechnology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728289/
https://www.ncbi.nlm.nih.gov/pubmed/32066215
http://dx.doi.org/10.4014/jmb.2001.01042