Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers

The development of RNA imaging strategies in live cells is essential to improve our understanding of their role in various cellular functions. We report an efficient RNA imaging method based on the CRISPR-dPspCas13b system with fluorescent RNA aptamers in sgRNA (CasFAS) in live cells. Using modified...

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Detalles Bibliográficos
Autores principales: Tang, Heng, Peng, Junran, Peng, Shuang, Wang, Qi, Jiang, Xin, Xue, Xiaocheng, Tao, Yanxin, Xiang, Limin, Ji, Quanjiang, Liu, Song-Mei, Weng, Xiaocheng, Zhou, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728512/
https://www.ncbi.nlm.nih.gov/pubmed/36540819
http://dx.doi.org/10.1039/d2sc04656c

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728512/
https://www.ncbi.nlm.nih.gov/pubmed/36540819
http://dx.doi.org/10.1039/d2sc04656c

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