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High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants
Polymerase chain reaction (PCR) variants requiring specific primer types are widely used in various PCR experiments, including generic PCR, inverse PCR, anchored PCR, and ARMS PCR. Few tools can be adapted for multiple PCR variants, and many tools select primers by filtration based on the given para...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729204/ https://www.ncbi.nlm.nih.gov/pubmed/36477468 http://dx.doi.org/10.1038/s41598-022-25561-z |