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High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants

Polymerase chain reaction (PCR) variants requiring specific primer types are widely used in various PCR experiments, including generic PCR, inverse PCR, anchored PCR, and ARMS PCR. Few tools can be adapted for multiple PCR variants, and many tools select primers by filtration based on the given para...

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Detalles Bibliográficos
Autores principales: Zeng, Huaping, Chen, Kexin, Ma, Chouxian, Zhu, Biyin, Chuan, Jun, Zhang, Shuan, Tang, Lin, Yang, Ting, Sun, Zhaohui, Yang, Xingkun, Wang, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729204/
https://www.ncbi.nlm.nih.gov/pubmed/36477468
http://dx.doi.org/10.1038/s41598-022-25561-z

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