Application of temperature-responsive HIS-tag fluorophores to differential scanning fluorimetry screening of small molecule libraries

Differential scanning fluorimetry is a rapid and economical biophysical technique used to monitor perturbations to protein structure during a thermal gradient, most often by detecting protein unfolding events through an environment-sensitive fluorophore. By employing an NTA-complexed fluorophore tha...

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Detalles Bibliográficos
Autores principales: Ronzetti, Michael H., Baljinnyam, Bolormaa, Itkin, Zina, Jain, Sankalp, Rai, Ganesha, Zakharov, Alexey V., Pal, Utpal, Simeonov, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Pharmacology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729254/
https://www.ncbi.nlm.nih.gov/pubmed/36506591
http://dx.doi.org/10.3389/fphar.2022.1040039

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729254/
https://www.ncbi.nlm.nih.gov/pubmed/36506591
http://dx.doi.org/10.3389/fphar.2022.1040039

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