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Generation of expandable cardiovascular progenitor cells from mouse and human fibroblasts via direct chemical reprogramming

Here, we present a protocol to reprogram mouse and human fibroblasts into expandable cardiovascular progenitor cells (CPCs) via a defined small-molecule treatment. We describe steps to prepare fibroblasts and generate the chemically induced CPCs (ciCPCs), followed by expansion and differentiation of...

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Detalles Bibliográficos
Autores principales: Wang, Jia, Wang, Qiyuan, Cao, Nan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730216/
https://www.ncbi.nlm.nih.gov/pubmed/36595887
http://dx.doi.org/10.1016/j.xpro.2022.101908