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Protocol for assessing phagocytosis activity in cultured primary murine microglia

In this protocol, we describe steps to assess inflammation-induced cell response in cultured primary murine microglia through the analysis of fluorescent bead phagocytosis. We detail primary murine mixed glial cell culture preparation followed by microglia-specific isolation. Further, we describe tr...

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Detalles Bibliográficos
Autores principales: Layman, Elsie, Parrott, Jennifer Michelle, Lee, Hye Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730218/
https://www.ncbi.nlm.nih.gov/pubmed/36595893
http://dx.doi.org/10.1016/j.xpro.2022.101881