An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin. The cell culture conditions have been optimized for inducing expression of keratinocyte differentiation markers. Cells are cultured in the absence o...

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Detalles Bibliográficos
Autores principales: Siriwach, Ratklao, Ngo, Anh Quynh, Narumiya, Shuh, Thumkeo, Dean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730219/
https://www.ncbi.nlm.nih.gov/pubmed/36595953
http://dx.doi.org/10.1016/j.xpro.2022.101906

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730219/
https://www.ncbi.nlm.nih.gov/pubmed/36595953
http://dx.doi.org/10.1016/j.xpro.2022.101906

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