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An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis
Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin. The cell culture conditions have been optimized for inducing expression of keratinocyte differentiation markers. Cells are cultured in the absence o...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730219/ https://www.ncbi.nlm.nih.gov/pubmed/36595953 http://dx.doi.org/10.1016/j.xpro.2022.101906 |