A robust pipeline for efficient knock-in of point mutations and epitope tags in zebrafish using fluorescent PCR based screening

BACKGROUND: Genome editing using CRISPR/Cas9 has become a powerful tool in zebrafish to generate targeted gene knockouts models. However, its use for targeted knock-in remains challenging due to inefficient homology directed repair (HDR) pathway in zebrafish, highlighting the need for efficient and...

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Detalles Bibliográficos
Autores principales: Carrington, Blake, Ramanagoudr-Bhojappa, Ramanagouda, Bresciani, Erica, Han, Tae-Un, Sood, Raman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730659/
https://www.ncbi.nlm.nih.gov/pubmed/36476416
http://dx.doi.org/10.1186/s12864-022-08971-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730659/
https://www.ncbi.nlm.nih.gov/pubmed/36476416
http://dx.doi.org/10.1186/s12864-022-08971-1

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