Cargando…
Fitness of calves born from in vitro-produced fresh and cryopreserved embryos
In cattle, vitrified/warmed (V/W) and frozen/thawed (F/T), in vitro-produced (IVP) embryos, differ in their physiology and survival from fresh embryos. In this study, we analyzed the effects of embryo cryopreservation techniques on the offspring. IVP embryos cultured with albumin and with or without...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730881/ https://www.ncbi.nlm.nih.gov/pubmed/36504844 http://dx.doi.org/10.3389/fvets.2022.1006995 |
_version_ | 1784845781717483520 |
---|---|
author | Gómez, Enrique Murillo, Antonio Carrocera, Susana Pérez-Jánez, Juan José Benedito, Jose Luis Martín-González, David Gimeno, Isabel |
author_facet | Gómez, Enrique Murillo, Antonio Carrocera, Susana Pérez-Jánez, Juan José Benedito, Jose Luis Martín-González, David Gimeno, Isabel |
author_sort | Gómez, Enrique |
collection | PubMed |
description | In cattle, vitrified/warmed (V/W) and frozen/thawed (F/T), in vitro-produced (IVP) embryos, differ in their physiology and survival from fresh embryos. In this study, we analyzed the effects of embryo cryopreservation techniques on the offspring. IVP embryos cultured with albumin and with or without 0.1% serum until Day 6, and thereafter in single culture without protein, were transferred to recipients on Day 7 as F/T, V/W, or fresh, resulting in N = 24, 14, and 13 calves, respectively. Calves were clinically examined at birth, and blood was analyzed before and after colostrum intake (Day 0), and subsequently on Day 15 and Day 30. On Day 0, calves from V/W and F/T embryos showed increased creatinine and capillary refill time (CRT) and reduced heartbeats. Calves from F/T embryos showed lower PCO(2), hemoglobin, and packed cell volume than calves from V/W embryos while V/W embryos led to calves with increased Na(+) levels. Colostrum effects did not differ between calves from fresh and cryopreserved embryos, indicating similar adaptive ability among calves. However, PCO(2) did not decrease in calves from V/W embryos after colostrum intake. Serum in culture led to calves with affected (P < 0.05) temperature, CRT, HCO [Formula: see text] , base excess (BE), TCO(2), creatinine, urea, and anion gap. On Day 15, the effects of embryo cryopreservation disappeared among calves. In contrast, Day 30 values were influenced by diarrhea appearance, mainly in calves from V/W embryos (i.e., lower values of TCO(2), HCO [Formula: see text] , and BE; and increased glucose, anion gap, and lactate), although with no more clinical compromise than calves from fresh and F/T embryos. Diarrhea affected PCO(2) and Na(+) in all groups. Embryo cryopreservation, and/or culture, yield metabolically different calves, including effects on protein and acid–base metabolism. |
format | Online Article Text |
id | pubmed-9730881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97308812022-12-09 Fitness of calves born from in vitro-produced fresh and cryopreserved embryos Gómez, Enrique Murillo, Antonio Carrocera, Susana Pérez-Jánez, Juan José Benedito, Jose Luis Martín-González, David Gimeno, Isabel Front Vet Sci Veterinary Science In cattle, vitrified/warmed (V/W) and frozen/thawed (F/T), in vitro-produced (IVP) embryos, differ in their physiology and survival from fresh embryos. In this study, we analyzed the effects of embryo cryopreservation techniques on the offspring. IVP embryos cultured with albumin and with or without 0.1% serum until Day 6, and thereafter in single culture without protein, were transferred to recipients on Day 7 as F/T, V/W, or fresh, resulting in N = 24, 14, and 13 calves, respectively. Calves were clinically examined at birth, and blood was analyzed before and after colostrum intake (Day 0), and subsequently on Day 15 and Day 30. On Day 0, calves from V/W and F/T embryos showed increased creatinine and capillary refill time (CRT) and reduced heartbeats. Calves from F/T embryos showed lower PCO(2), hemoglobin, and packed cell volume than calves from V/W embryos while V/W embryos led to calves with increased Na(+) levels. Colostrum effects did not differ between calves from fresh and cryopreserved embryos, indicating similar adaptive ability among calves. However, PCO(2) did not decrease in calves from V/W embryos after colostrum intake. Serum in culture led to calves with affected (P < 0.05) temperature, CRT, HCO [Formula: see text] , base excess (BE), TCO(2), creatinine, urea, and anion gap. On Day 15, the effects of embryo cryopreservation disappeared among calves. In contrast, Day 30 values were influenced by diarrhea appearance, mainly in calves from V/W embryos (i.e., lower values of TCO(2), HCO [Formula: see text] , and BE; and increased glucose, anion gap, and lactate), although with no more clinical compromise than calves from fresh and F/T embryos. Diarrhea affected PCO(2) and Na(+) in all groups. Embryo cryopreservation, and/or culture, yield metabolically different calves, including effects on protein and acid–base metabolism. Frontiers Media S.A. 2022-11-24 /pmc/articles/PMC9730881/ /pubmed/36504844 http://dx.doi.org/10.3389/fvets.2022.1006995 Text en Copyright © 2022 Gómez, Murillo, Carrocera, Pérez-Jánez, Benedito, Martín-González and Gimeno. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Gómez, Enrique Murillo, Antonio Carrocera, Susana Pérez-Jánez, Juan José Benedito, Jose Luis Martín-González, David Gimeno, Isabel Fitness of calves born from in vitro-produced fresh and cryopreserved embryos |
title | Fitness of calves born from in vitro-produced fresh and cryopreserved embryos |
title_full | Fitness of calves born from in vitro-produced fresh and cryopreserved embryos |
title_fullStr | Fitness of calves born from in vitro-produced fresh and cryopreserved embryos |
title_full_unstemmed | Fitness of calves born from in vitro-produced fresh and cryopreserved embryos |
title_short | Fitness of calves born from in vitro-produced fresh and cryopreserved embryos |
title_sort | fitness of calves born from in vitro-produced fresh and cryopreserved embryos |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730881/ https://www.ncbi.nlm.nih.gov/pubmed/36504844 http://dx.doi.org/10.3389/fvets.2022.1006995 |
work_keys_str_mv | AT gomezenrique fitnessofcalvesbornfrominvitroproducedfreshandcryopreservedembryos AT murilloantonio fitnessofcalvesbornfrominvitroproducedfreshandcryopreservedembryos AT carrocerasusana fitnessofcalvesbornfrominvitroproducedfreshandcryopreservedembryos AT perezjanezjuanjose fitnessofcalvesbornfrominvitroproducedfreshandcryopreservedembryos AT beneditojoseluis fitnessofcalvesbornfrominvitroproducedfreshandcryopreservedembryos AT martingonzalezdavid fitnessofcalvesbornfrominvitroproducedfreshandcryopreservedembryos AT gimenoisabel fitnessofcalvesbornfrominvitroproducedfreshandcryopreservedembryos |