Protocol to identify small molecules promoting rat and mouse cardiomyocyte proliferation based on the FUCCI and MADM reporters

Discovery of small molecules promoting cardiomyocyte proliferation is important for heart regeneration and related heart disease. Here, we describe a protocol to isolate neonatal rat and mouse cardiomyocytes, infect cardiomyocytes with Tnnt2-mAG-hGeminin (1/110) or Tnnt2-Cre adenovirus, and identify...

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Detalles Bibliográficos
Autores principales: Zheng, Lixia, Wang, Zihao, Du, Jianyong, Zhu, Xiaojun, Xiong, Jing-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732407/
https://www.ncbi.nlm.nih.gov/pubmed/36595956
http://dx.doi.org/10.1016/j.xpro.2022.101903

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732407/
https://www.ncbi.nlm.nih.gov/pubmed/36595956
http://dx.doi.org/10.1016/j.xpro.2022.101903

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