Cargando…

Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae

Single-molecule tracking (SMT) is a powerful approach to quantify the biophysical parameters of protein dynamics in live cells. Here, we describe a protocol for SMT in live cells of the budding yeast Saccharomyces cerevisiae. We detail how to genetically engineer yeast strains for SMT, how to set up...

Descripción completa

Detalles Bibliográficos
Autores principales: Podh, Nitesh Kumar, Das, Ayan, Dey, Partha, Paliwal, Sheetal, Mehta, Gunjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732415/
https://www.ncbi.nlm.nih.gov/pubmed/36595957
http://dx.doi.org/10.1016/j.xpro.2022.101900