Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae

Single-molecule tracking (SMT) is a powerful approach to quantify the biophysical parameters of protein dynamics in live cells. Here, we describe a protocol for SMT in live cells of the budding yeast Saccharomyces cerevisiae. We detail how to genetically engineer yeast strains for SMT, how to set up...

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Detalles Bibliográficos
Autores principales: Podh, Nitesh Kumar, Das, Ayan, Dey, Partha, Paliwal, Sheetal, Mehta, Gunjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732415/
https://www.ncbi.nlm.nih.gov/pubmed/36595957
http://dx.doi.org/10.1016/j.xpro.2022.101900

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732415/
https://www.ncbi.nlm.nih.gov/pubmed/36595957
http://dx.doi.org/10.1016/j.xpro.2022.101900

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