IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction

BACKGROUND: Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amounts of high-quality genomic DNA. Other PCR-based techniques are either inaccurat...

Descripción completa

Detalles Bibliográficos
Autores principales: De Saeger, Jonas, Park, Jihae, Thoris, Kai, De Bruyn, Charlotte, Chung, Hoo Sun, Inzé, Dirk, Depuydt, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732982/
https://www.ncbi.nlm.nih.gov/pubmed/36494670
http://dx.doi.org/10.1186/s13007-022-00965-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732982/
https://www.ncbi.nlm.nih.gov/pubmed/36494670
http://dx.doi.org/10.1186/s13007-022-00965-0

Ejemplares similares