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IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction
BACKGROUND: Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amounts of high-quality genomic DNA. Other PCR-based techniques are either inaccurat...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732982/ https://www.ncbi.nlm.nih.gov/pubmed/36494670 http://dx.doi.org/10.1186/s13007-022-00965-0 |