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Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis

Chlamydomonas reinhardtii is an important model organism for the study of many cellular processes, and protein tagging is an increasingly indispensable tool for these studies. To circumvent the disadvantages of conventional approaches in creating a tagged cell line, which involve transforming either...

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Detalles Bibliográficos
Autores principales: Hou, Yuqing, Cheng, Xi, Witman, George B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733891/
https://www.ncbi.nlm.nih.gov/pubmed/36490276
http://dx.doi.org/10.1371/journal.pone.0278972