Cargando…

Combined Effects of Lycopene and Metformin on Decreasing Oxidative Stress by Triggering Endogenous Antioxidant Defenses in Diet-Induced Obese Mice

Since lycopene has antioxidant activity, its combination with metformin may be useful to contrast diabetic complications related to oxidative stress. This study aimed to investigate the effects of metformin combined with lycopene on high-fat diet (HFD)-induced obese mice. Seventy-two C57BL-6J mice w...

Descripción completa

Detalles Bibliográficos
Autores principales: Motta, Bruno Pereira, Pinheiro, Camila Graça, Figueiredo, Ingrid Delbone, Cardoso, Felipe Nunes, Oliveira, Juliana Oriel, Machado, Rachel Temperani Amaral, da Silva, Patrícia Bento, Chorilli, Marlus, Brunetti, Iguatemy Lourenço, Baviera, Amanda Martins
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737677/
https://www.ncbi.nlm.nih.gov/pubmed/36500596
http://dx.doi.org/10.3390/molecules27238503
_version_ 1784847350483648512
author Motta, Bruno Pereira
Pinheiro, Camila Graça
Figueiredo, Ingrid Delbone
Cardoso, Felipe Nunes
Oliveira, Juliana Oriel
Machado, Rachel Temperani Amaral
da Silva, Patrícia Bento
Chorilli, Marlus
Brunetti, Iguatemy Lourenço
Baviera, Amanda Martins
author_facet Motta, Bruno Pereira
Pinheiro, Camila Graça
Figueiredo, Ingrid Delbone
Cardoso, Felipe Nunes
Oliveira, Juliana Oriel
Machado, Rachel Temperani Amaral
da Silva, Patrícia Bento
Chorilli, Marlus
Brunetti, Iguatemy Lourenço
Baviera, Amanda Martins
author_sort Motta, Bruno Pereira
collection PubMed
description Since lycopene has antioxidant activity, its combination with metformin may be useful to contrast diabetic complications related to oxidative stress. This study aimed to investigate the effects of metformin combined with lycopene on high-fat diet (HFD)-induced obese mice. Seventy-two C57BL-6J mice were divided into six groups: C (control diet-fed mice), H (HFD-fed mice for 17 weeks), H-V (HFD-fed mice treated with vehicle), H-M (HFD-fed mice treated with 50 mg/kg metformin), H-L (HFD-fed mice treated with 45 mg/kg lycopene), and H-ML (HFD-fed mice treated with 50 mg/kg metformin + 45 mg/kg lycopene). Treatments were administered for 8 weeks. Glucose tolerance, insulin sensitivity, fluorescent AGEs (advanced glycation end products), TBARS (thiobarbituric acid-reactive substances), and activities of antioxidant enzymes paraoxonase-1 (PON-1; plasma), superoxide dismutase, catalase and glutathione peroxidase (liver and kidneys) were determined. Metformin plus lycopene reduced body weight; improved insulin sensitivity and glucose tolerance; and decreased AGEs and TBARS in plasma, liver and kidneys. Combined therapy significantly increased the activities of antioxidant enzymes, mainly PON-1. Lycopene combined with metformin improved insulin resistance and glucose tolerance, and caused further increases in endogenous antioxidant defenses, arising as a promising therapeutic strategy for combating diabetic complications resulting from glycoxidative stress.
format Online
Article
Text
id pubmed-9737677
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97376772022-12-11 Combined Effects of Lycopene and Metformin on Decreasing Oxidative Stress by Triggering Endogenous Antioxidant Defenses in Diet-Induced Obese Mice Motta, Bruno Pereira Pinheiro, Camila Graça Figueiredo, Ingrid Delbone Cardoso, Felipe Nunes Oliveira, Juliana Oriel Machado, Rachel Temperani Amaral da Silva, Patrícia Bento Chorilli, Marlus Brunetti, Iguatemy Lourenço Baviera, Amanda Martins Molecules Article Since lycopene has antioxidant activity, its combination with metformin may be useful to contrast diabetic complications related to oxidative stress. This study aimed to investigate the effects of metformin combined with lycopene on high-fat diet (HFD)-induced obese mice. Seventy-two C57BL-6J mice were divided into six groups: C (control diet-fed mice), H (HFD-fed mice for 17 weeks), H-V (HFD-fed mice treated with vehicle), H-M (HFD-fed mice treated with 50 mg/kg metformin), H-L (HFD-fed mice treated with 45 mg/kg lycopene), and H-ML (HFD-fed mice treated with 50 mg/kg metformin + 45 mg/kg lycopene). Treatments were administered for 8 weeks. Glucose tolerance, insulin sensitivity, fluorescent AGEs (advanced glycation end products), TBARS (thiobarbituric acid-reactive substances), and activities of antioxidant enzymes paraoxonase-1 (PON-1; plasma), superoxide dismutase, catalase and glutathione peroxidase (liver and kidneys) were determined. Metformin plus lycopene reduced body weight; improved insulin sensitivity and glucose tolerance; and decreased AGEs and TBARS in plasma, liver and kidneys. Combined therapy significantly increased the activities of antioxidant enzymes, mainly PON-1. Lycopene combined with metformin improved insulin resistance and glucose tolerance, and caused further increases in endogenous antioxidant defenses, arising as a promising therapeutic strategy for combating diabetic complications resulting from glycoxidative stress. MDPI 2022-12-03 /pmc/articles/PMC9737677/ /pubmed/36500596 http://dx.doi.org/10.3390/molecules27238503 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Motta, Bruno Pereira
Pinheiro, Camila Graça
Figueiredo, Ingrid Delbone
Cardoso, Felipe Nunes
Oliveira, Juliana Oriel
Machado, Rachel Temperani Amaral
da Silva, Patrícia Bento
Chorilli, Marlus
Brunetti, Iguatemy Lourenço
Baviera, Amanda Martins
Combined Effects of Lycopene and Metformin on Decreasing Oxidative Stress by Triggering Endogenous Antioxidant Defenses in Diet-Induced Obese Mice
title Combined Effects of Lycopene and Metformin on Decreasing Oxidative Stress by Triggering Endogenous Antioxidant Defenses in Diet-Induced Obese Mice
title_full Combined Effects of Lycopene and Metformin on Decreasing Oxidative Stress by Triggering Endogenous Antioxidant Defenses in Diet-Induced Obese Mice
title_fullStr Combined Effects of Lycopene and Metformin on Decreasing Oxidative Stress by Triggering Endogenous Antioxidant Defenses in Diet-Induced Obese Mice
title_full_unstemmed Combined Effects of Lycopene and Metformin on Decreasing Oxidative Stress by Triggering Endogenous Antioxidant Defenses in Diet-Induced Obese Mice
title_short Combined Effects of Lycopene and Metformin on Decreasing Oxidative Stress by Triggering Endogenous Antioxidant Defenses in Diet-Induced Obese Mice
title_sort combined effects of lycopene and metformin on decreasing oxidative stress by triggering endogenous antioxidant defenses in diet-induced obese mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737677/
https://www.ncbi.nlm.nih.gov/pubmed/36500596
http://dx.doi.org/10.3390/molecules27238503
work_keys_str_mv AT mottabrunopereira combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice
AT pinheirocamilagraca combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice
AT figueiredoingriddelbone combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice
AT cardosofelipenunes combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice
AT oliveirajulianaoriel combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice
AT machadoracheltemperaniamaral combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice
AT dasilvapatriciabento combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice
AT chorillimarlus combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice
AT brunettiiguatemylourenco combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice
AT bavieraamandamartins combinedeffectsoflycopeneandmetforminondecreasingoxidativestressbytriggeringendogenousantioxidantdefensesindietinducedobesemice