An Improved Real-Time Viability PCR Assay to Detect Salmonella in a Culture-Independent Era

Viability PCR (vPCR) uses a DNA intercalating dye to irreversibly bind double-stranded DNA from organisms with compromised cell membranes. This allows the selective amplification of DNA from intact cells. An optimized vPCR protocol should minimize false positives (DNA from compromised cells not full...

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Detalles Bibliográficos
Autores principales: Thilakarathna, Surangi H., Stokowski, Taryn, Chui, Linda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738789/
https://www.ncbi.nlm.nih.gov/pubmed/36499040
http://dx.doi.org/10.3390/ijms232314708

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738789/
https://www.ncbi.nlm.nih.gov/pubmed/36499040
http://dx.doi.org/10.3390/ijms232314708

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