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An Improved Real-Time Viability PCR Assay to Detect Salmonella in a Culture-Independent Era
Viability PCR (vPCR) uses a DNA intercalating dye to irreversibly bind double-stranded DNA from organisms with compromised cell membranes. This allows the selective amplification of DNA from intact cells. An optimized vPCR protocol should minimize false positives (DNA from compromised cells not full...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738789/ https://www.ncbi.nlm.nih.gov/pubmed/36499040 http://dx.doi.org/10.3390/ijms232314708 |