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Development and Validation of a Method for Determination of 43 Antimicrobial Drugs in Western-Style Pork Products by UPLC-MS/MS with the Aid of Experimental Design

Western-style pork products have attracted many modern urban consumers, and these products have rapidly entered the Chinese market. The current hazard analysis of processed meat products mainly focuses on processing hazards (PAHs, microorganisms, and food additives), with less attention to veterinar...

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Autores principales: Yu, Xiaoxuan, Wu, Xingqiang, Xie, Yujie, Tong, Kaixuan, Wang, Minglin, Li, Jianhui, Fan, Chunlin, Chen, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739473/
https://www.ncbi.nlm.nih.gov/pubmed/36500374
http://dx.doi.org/10.3390/molecules27238283
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author Yu, Xiaoxuan
Wu, Xingqiang
Xie, Yujie
Tong, Kaixuan
Wang, Minglin
Li, Jianhui
Fan, Chunlin
Chen, Hui
author_facet Yu, Xiaoxuan
Wu, Xingqiang
Xie, Yujie
Tong, Kaixuan
Wang, Minglin
Li, Jianhui
Fan, Chunlin
Chen, Hui
author_sort Yu, Xiaoxuan
collection PubMed
description Western-style pork products have attracted many modern urban consumers, and these products have rapidly entered the Chinese market. The current hazard analysis of processed meat products mainly focuses on processing hazards (PAHs, microorganisms, and food additives), with less attention to veterinary drug residues. According to the survey results, the residues of antimicrobial drugs (sulfonamides and quinolones) in pork and its products in China are a severe problem, which may cause metabolic reactions, toxic effects, or enhance drug resistance. This study applied a modified QuEChERS method combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MSMS) to develop a rapid and sensitive method for determining antimicrobial drugs in bacon and ham was successfully evaluated methodologically by EU 2002/657/EC. This study used a three-level, three-factor Box–Behnken design (BBD) to optimize the QuEChERS method by response surface methodology. The excellent linearity of the calibration curve was shown in the corresponding concentration range with a coefficient of determination greater than 0.99. The values of decision limit (CCα) and detection capability (CCβ) were in the range of 10.9–31.3 μg/kg and 11.8–52.5 μg/kg, respectively. The method successfully detected two trace levels of antimicrobial drugs in commercially available samples, including sulfadiazine and moxifloxacin.
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spelling pubmed-97394732022-12-11 Development and Validation of a Method for Determination of 43 Antimicrobial Drugs in Western-Style Pork Products by UPLC-MS/MS with the Aid of Experimental Design Yu, Xiaoxuan Wu, Xingqiang Xie, Yujie Tong, Kaixuan Wang, Minglin Li, Jianhui Fan, Chunlin Chen, Hui Molecules Article Western-style pork products have attracted many modern urban consumers, and these products have rapidly entered the Chinese market. The current hazard analysis of processed meat products mainly focuses on processing hazards (PAHs, microorganisms, and food additives), with less attention to veterinary drug residues. According to the survey results, the residues of antimicrobial drugs (sulfonamides and quinolones) in pork and its products in China are a severe problem, which may cause metabolic reactions, toxic effects, or enhance drug resistance. This study applied a modified QuEChERS method combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MSMS) to develop a rapid and sensitive method for determining antimicrobial drugs in bacon and ham was successfully evaluated methodologically by EU 2002/657/EC. This study used a three-level, three-factor Box–Behnken design (BBD) to optimize the QuEChERS method by response surface methodology. The excellent linearity of the calibration curve was shown in the corresponding concentration range with a coefficient of determination greater than 0.99. The values of decision limit (CCα) and detection capability (CCβ) were in the range of 10.9–31.3 μg/kg and 11.8–52.5 μg/kg, respectively. The method successfully detected two trace levels of antimicrobial drugs in commercially available samples, including sulfadiazine and moxifloxacin. MDPI 2022-11-28 /pmc/articles/PMC9739473/ /pubmed/36500374 http://dx.doi.org/10.3390/molecules27238283 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Xiaoxuan
Wu, Xingqiang
Xie, Yujie
Tong, Kaixuan
Wang, Minglin
Li, Jianhui
Fan, Chunlin
Chen, Hui
Development and Validation of a Method for Determination of 43 Antimicrobial Drugs in Western-Style Pork Products by UPLC-MS/MS with the Aid of Experimental Design
title Development and Validation of a Method for Determination of 43 Antimicrobial Drugs in Western-Style Pork Products by UPLC-MS/MS with the Aid of Experimental Design
title_full Development and Validation of a Method for Determination of 43 Antimicrobial Drugs in Western-Style Pork Products by UPLC-MS/MS with the Aid of Experimental Design
title_fullStr Development and Validation of a Method for Determination of 43 Antimicrobial Drugs in Western-Style Pork Products by UPLC-MS/MS with the Aid of Experimental Design
title_full_unstemmed Development and Validation of a Method for Determination of 43 Antimicrobial Drugs in Western-Style Pork Products by UPLC-MS/MS with the Aid of Experimental Design
title_short Development and Validation of a Method for Determination of 43 Antimicrobial Drugs in Western-Style Pork Products by UPLC-MS/MS with the Aid of Experimental Design
title_sort development and validation of a method for determination of 43 antimicrobial drugs in western-style pork products by uplc-ms/ms with the aid of experimental design
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739473/
https://www.ncbi.nlm.nih.gov/pubmed/36500374
http://dx.doi.org/10.3390/molecules27238283
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