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BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale

Progoitrin (2-hydroxy-3-butenyl glucosinolate, PRO) is the main source of bitterness of Brassica plants. Research on the biosynthesis of PRO glucosinolate can aid the understanding of the nutritional value in Brassica plants. In this study, four ODD genes likely involved in PRO biosynthesis were clo...

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Autores principales: Wu, Shuanghua, Zhang, Ting, Wang, Yudan, Chen, Muxi, Yang, Jianguo, Li, Fei, Deng, Ying, Zhu, Zhangsheng, Lei, Jianjun, Chen, Guoju, Cao, Bihao, Chen, Changming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739482/
https://www.ncbi.nlm.nih.gov/pubmed/36499110
http://dx.doi.org/10.3390/ijms232314781
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author Wu, Shuanghua
Zhang, Ting
Wang, Yudan
Chen, Muxi
Yang, Jianguo
Li, Fei
Deng, Ying
Zhu, Zhangsheng
Lei, Jianjun
Chen, Guoju
Cao, Bihao
Chen, Changming
author_facet Wu, Shuanghua
Zhang, Ting
Wang, Yudan
Chen, Muxi
Yang, Jianguo
Li, Fei
Deng, Ying
Zhu, Zhangsheng
Lei, Jianjun
Chen, Guoju
Cao, Bihao
Chen, Changming
author_sort Wu, Shuanghua
collection PubMed
description Progoitrin (2-hydroxy-3-butenyl glucosinolate, PRO) is the main source of bitterness of Brassica plants. Research on the biosynthesis of PRO glucosinolate can aid the understanding of the nutritional value in Brassica plants. In this study, four ODD genes likely involved in PRO biosynthesis were cloned from Chinese kale. These four genes, designated as BocODD1–4, shared 75–82% similarities with the ODD sequence of Arabidopsis. The sequences of these four BocODDs were analyzed, and BocODD1 and BocODD2 were chosen for further study. The gene BocODD1,2 showed the highest expression levels in the roots, followed by the leaves, flowers, and stems, which is in accordance with the trend of the PRO content in the same tissues. Both the expression levels of BocODD1,2 and the content of PRO were significantly induced by high- and low-temperature treatments. The function of BocODDs involved in PRO biosynthesis was identified. Compared with the wild type, the content of PRO was increased twofold in the over-expressing BocODD1 or BocODD2 plants. Meanwhile, the content of PRO was decreased in the BocODD1 or BocODD2 RNAi lines more than twofold compared to the wildtype plants. These results suggested that BocODD1 and BocODD2 may play important roles in the biosynthesis of PRO glucosinolate in Chinese kale.
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spelling pubmed-97394822022-12-11 BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale Wu, Shuanghua Zhang, Ting Wang, Yudan Chen, Muxi Yang, Jianguo Li, Fei Deng, Ying Zhu, Zhangsheng Lei, Jianjun Chen, Guoju Cao, Bihao Chen, Changming Int J Mol Sci Article Progoitrin (2-hydroxy-3-butenyl glucosinolate, PRO) is the main source of bitterness of Brassica plants. Research on the biosynthesis of PRO glucosinolate can aid the understanding of the nutritional value in Brassica plants. In this study, four ODD genes likely involved in PRO biosynthesis were cloned from Chinese kale. These four genes, designated as BocODD1–4, shared 75–82% similarities with the ODD sequence of Arabidopsis. The sequences of these four BocODDs were analyzed, and BocODD1 and BocODD2 were chosen for further study. The gene BocODD1,2 showed the highest expression levels in the roots, followed by the leaves, flowers, and stems, which is in accordance with the trend of the PRO content in the same tissues. Both the expression levels of BocODD1,2 and the content of PRO were significantly induced by high- and low-temperature treatments. The function of BocODDs involved in PRO biosynthesis was identified. Compared with the wild type, the content of PRO was increased twofold in the over-expressing BocODD1 or BocODD2 plants. Meanwhile, the content of PRO was decreased in the BocODD1 or BocODD2 RNAi lines more than twofold compared to the wildtype plants. These results suggested that BocODD1 and BocODD2 may play important roles in the biosynthesis of PRO glucosinolate in Chinese kale. MDPI 2022-11-26 /pmc/articles/PMC9739482/ /pubmed/36499110 http://dx.doi.org/10.3390/ijms232314781 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Shuanghua
Zhang, Ting
Wang, Yudan
Chen, Muxi
Yang, Jianguo
Li, Fei
Deng, Ying
Zhu, Zhangsheng
Lei, Jianjun
Chen, Guoju
Cao, Bihao
Chen, Changming
BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale
title BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale
title_full BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale
title_fullStr BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale
title_full_unstemmed BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale
title_short BocODD1 and BocODD2 Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale
title_sort bocodd1 and bocodd2 regulate the biosynthesis of progoitrin glucosinolate in chinese kale
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739482/
https://www.ncbi.nlm.nih.gov/pubmed/36499110
http://dx.doi.org/10.3390/ijms232314781
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