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Effects of heat stress on growth performance, carcass traits, serum metabolism, and intestinal microflora of meat rabbits

To investigate the effects of heat stress on meat rabbits, we assigned 80 rabbits to the moderate temperature group (24 ± 1°C; Control group) and the continuous high-temperature group (HT group), then monitored the effects using growth performance, carcass characteristics, biochemical assays, UPLC–M...

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Autores principales: Liu, Hongli, Zhang, Bin, Li, Fan, Liu, Lei, Yang, Tongao, Zhang, Haihua, Li, Fuchang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9743647/
https://www.ncbi.nlm.nih.gov/pubmed/36519173
http://dx.doi.org/10.3389/fmicb.2022.998095
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author Liu, Hongli
Zhang, Bin
Li, Fan
Liu, Lei
Yang, Tongao
Zhang, Haihua
Li, Fuchang
author_facet Liu, Hongli
Zhang, Bin
Li, Fan
Liu, Lei
Yang, Tongao
Zhang, Haihua
Li, Fuchang
author_sort Liu, Hongli
collection PubMed
description To investigate the effects of heat stress on meat rabbits, we assigned 80 rabbits to the moderate temperature group (24 ± 1°C; Control group) and the continuous high-temperature group (HT group), then monitored the effects using growth performance, carcass characteristics, biochemical assays, UPLC–MS/MS-based metabolomics, and microbiome. The results showed that after continuous high-temperature exposure, the average daily gain, average daily feed intake, and thymus index were significantly decreased (p < 0.05). Contents of HSP70, ALP, and Cortisol in serum were significantly increased, while TP, GLU, T3, and T4 were significantly decreased (p < 0.05). Nine kinds of differential metabolites were screened by serum metabolomics, which can be used as biomarkers of heat stress in meat rabbits. The selected differential metabolites were analyzed by KEGG annotation and enrichment analysis. The results showed that 14 pathways affected by heat stress were identified by KEGG pathway enrichment analysis, including Sphingolipid metabolism, Pyrimidine metabolism, Citrate cycle (TCA cycle)), aminoacyl-tRNA biosynthesis, and so on. The analysis of the effect of heat stress on the cecal microflora of meat rabbits showed that the abundance of cecal Proteus in the HT group was significantly higher than that in the moderate Control group. The number of Candidatus-saccharimonas in the cecum microflora was significantly higher than that in the moderate temperature group (p < 0.05) which may be related to inflammatory diseases in the heat stress group. These findings indicated that the heat-stressed rabbits were in negative energy balance, which affected protein metabolism, and subsequently affected growth performance and carcass characteristics.
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spelling pubmed-97436472022-12-13 Effects of heat stress on growth performance, carcass traits, serum metabolism, and intestinal microflora of meat rabbits Liu, Hongli Zhang, Bin Li, Fan Liu, Lei Yang, Tongao Zhang, Haihua Li, Fuchang Front Microbiol Microbiology To investigate the effects of heat stress on meat rabbits, we assigned 80 rabbits to the moderate temperature group (24 ± 1°C; Control group) and the continuous high-temperature group (HT group), then monitored the effects using growth performance, carcass characteristics, biochemical assays, UPLC–MS/MS-based metabolomics, and microbiome. The results showed that after continuous high-temperature exposure, the average daily gain, average daily feed intake, and thymus index were significantly decreased (p < 0.05). Contents of HSP70, ALP, and Cortisol in serum were significantly increased, while TP, GLU, T3, and T4 were significantly decreased (p < 0.05). Nine kinds of differential metabolites were screened by serum metabolomics, which can be used as biomarkers of heat stress in meat rabbits. The selected differential metabolites were analyzed by KEGG annotation and enrichment analysis. The results showed that 14 pathways affected by heat stress were identified by KEGG pathway enrichment analysis, including Sphingolipid metabolism, Pyrimidine metabolism, Citrate cycle (TCA cycle)), aminoacyl-tRNA biosynthesis, and so on. The analysis of the effect of heat stress on the cecal microflora of meat rabbits showed that the abundance of cecal Proteus in the HT group was significantly higher than that in the moderate Control group. The number of Candidatus-saccharimonas in the cecum microflora was significantly higher than that in the moderate temperature group (p < 0.05) which may be related to inflammatory diseases in the heat stress group. These findings indicated that the heat-stressed rabbits were in negative energy balance, which affected protein metabolism, and subsequently affected growth performance and carcass characteristics. Frontiers Media S.A. 2022-11-28 /pmc/articles/PMC9743647/ /pubmed/36519173 http://dx.doi.org/10.3389/fmicb.2022.998095 Text en Copyright © 2022 Liu, Zhang, Li, Liu, Yang, Zhang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Hongli
Zhang, Bin
Li, Fan
Liu, Lei
Yang, Tongao
Zhang, Haihua
Li, Fuchang
Effects of heat stress on growth performance, carcass traits, serum metabolism, and intestinal microflora of meat rabbits
title Effects of heat stress on growth performance, carcass traits, serum metabolism, and intestinal microflora of meat rabbits
title_full Effects of heat stress on growth performance, carcass traits, serum metabolism, and intestinal microflora of meat rabbits
title_fullStr Effects of heat stress on growth performance, carcass traits, serum metabolism, and intestinal microflora of meat rabbits
title_full_unstemmed Effects of heat stress on growth performance, carcass traits, serum metabolism, and intestinal microflora of meat rabbits
title_short Effects of heat stress on growth performance, carcass traits, serum metabolism, and intestinal microflora of meat rabbits
title_sort effects of heat stress on growth performance, carcass traits, serum metabolism, and intestinal microflora of meat rabbits
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9743647/
https://www.ncbi.nlm.nih.gov/pubmed/36519173
http://dx.doi.org/10.3389/fmicb.2022.998095
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