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Investigation on the applicability of a long-range reverse-transcription quantitative polymerase chain reaction assay for the rapid detection of active viruses

BACKGROUND: Although conventional polymerase chain reaction (PCR) methods are widely used in diagnosis, the titer of the pathogenic virus is difficult to determine based on the PCR. In our prior report, a long-range reverse-transcription quantitative PCR (LR-RT-qPCR) assay was developed to assess th...

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Detalles Bibliográficos
Autores principales: Yasuura, Masato, Nakaya, Yuki, Ashiba, Hiroki, Fukuda, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9743722/
https://www.ncbi.nlm.nih.gov/pubmed/36510144
http://dx.doi.org/10.1186/s12866-022-02723-7