Comparison between relative and absolute quantitative real-time PCR applied to single-cell analyses: Transcriptional levels in a key neuron for long-term memory in the pond snail

Quantitative real-time PCR (qPCR) is a powerful method for measuring nucleic acid levels and quantifying mRNA levels, even in single cells. In the present study, we compared the results of single-cell qPCR obtained by different quantification methods (relative and absolute) and different reverse tra...

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Detalles Bibliográficos
Autores principales: Hatakeyama, Dai, Chikamoto, Nozomi, Fujimoto, Kanta, Kitahashi, Takashi, Ito, Etsuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9744327/
https://www.ncbi.nlm.nih.gov/pubmed/36508476
http://dx.doi.org/10.1371/journal.pone.0279017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9744327/
https://www.ncbi.nlm.nih.gov/pubmed/36508476
http://dx.doi.org/10.1371/journal.pone.0279017

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