Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by...

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Detalles Bibliográficos
Autores principales: Trumpp, Michael, Oliveras, Anna, Gonschior, Hannes, Ast, Julia, Hodson, David J., Knaus, Petra, Lehmann, Martin, Birol, Melissa, Broichhagen, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745879/
https://www.ncbi.nlm.nih.gov/pubmed/36427021
http://dx.doi.org/10.1039/d2cc04823j

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745879/
https://www.ncbi.nlm.nih.gov/pubmed/36427021
http://dx.doi.org/10.1039/d2cc04823j

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